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Study of fluorescence lifetime and spectral changes of nanoparticles in cell biology
Pelc, Pavel ; Janoušek, Oto (referee) ; Čmiel, Vratislav (advisor)
This work deals with the study of fluorescence lifetime and spectral changes of nanoparticles in cell biology. It describes the principle of fluorescence, fluorescence microscopy and laser confocal microscope Leica TCS SP8. The classic FLIM method, the Lambda Square mapping and the division of nanoparticles are introduced there. In the practical part, the created program for the evaluation of fluorescence lifetime and spectral changes is described. The program can show two-dimensional lambda maps, the fluorescence lifetime and spectral shift in the space area. In the final part of the thesis, an experiment with rhodamine nanoparticles is carried out and it is evaluated using the created program and then discussed.
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CDMA Network Capacity
Pasečný, Jan ; Kejík, Petr (referee) ; Hanus, Stanislav (advisor)
The Bachelor thesis deals with determining which factors influence the capacity of CDMA network. This type of network is now being used worldwide due to its huge increase in capacity when compared to other transmissions systems. The work contains summary of basic parameters of single-cell and multi-cell systems with reference to the capacity. Obtained results are assesed and compared at the end of the work.
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Localization of the caller in emergency medicine information system
Maleňák, Filip ; Sekora, Jiří (referee) ; Rozman, Jiří (advisor)
Aim of this bachelor’s thesis is to analyze methods that are used for localization of the caller during emergency call to 155 and the importance of an accurate localization during the rescue operation. Describe other methods that can be used for localization of the caller and draft of the solution for use these methods to enhanced localization of the caller. Emergency line is presented by the module of emergency operation center in emergency medical service information system. Proposed solution for enhanced localization of the caller is given by the iPhone application iSOS. The application was designed on requirement of simplicity and maximum efficiency of caller localization in emergency situation.
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Band Gap - accurate voltage reference
Bubla, Jiří ; Petržela, Jiří (referee) ; Kolka, Zdeněk (advisor)
This diploma thesis is specialized on a design of a high accuracy voltage reference Bandgap. A very low temperature coefficient and output voltage approx. 1,205V are the main features of this circuit. The paper contains a derivation of the Bandgap principle, examples of realizations of the circuits and methods of compensation temperature dependence and manufacture process, design of Brokaw and Gilbert reference, design of a testchip and measurement results.
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Optical Method to Evaluate the Contractile Function of Isolated Cardiac Myocytes
Kopečný, Josef ; Harabiš, Vratislav (referee) ; Čmiel, Vratislav (advisor)
In this master´s thesis we will firstly focused on the description of a cyte in terms of structure as well as in terms of electrical and chemical processes. We will examine a processes, which make a contraction and the processes on cyte membrane. We will also analyse image processing methods and methods for measuring the contraction. The block diagram will be designed and requirements for the measuring platform will be specified. The programme will be realized in LabWIEV programming language.
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Ferrous nanoparts localization in inner organelles
Solař, Jan ; Janoušek, Oto (referee) ; Čmiel, Vratislav (advisor)
This bachelor‘s thesis describes a behavior of iron nanoparticles in human mesenchymal stem cells. First section deals with methods of fluorescent labelling of organelles and nanoparticles and settings of confocal microscope for their detection. Next section describes the iron nanoparticles metabolism and accumulation in cells. Finally, there is a section about the developement of the software utility for the localization and for the quantitative analysis of the fluorescence organelles and nanoparticles inside the living cells.
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The effect of electromagnetic fields on cell structure
Urbánek, Jiří ; Fiala, Pavel (referee) ; Vlachová Hutová, Eliška (advisor)
The main concern of the work is researching the influence of electromagnetic field on cellular structures. Cellular structures is a general term for all the organelles placed in a cell. In our 2D model we take into consideration just the fundamental organelles such as cell membrane, cytoplasm and cell nucleus. The aim of the work was to get acquainted with the outer environment into which we had placed the 2D model of the cell and on which we had applied the magnetic field. The work contains the results gained by numeric modelling in the physical programme COMSOL. These results prove the theoretical presumption.
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Application for simulation of action potentials and membrane currents
Klouba, Lukáš ; Čmiel, Vratislav (referee) ; Chmelíková, Larisa (advisor)
This thesis deals with the basic electronic processes in the cell membrane, the methods of measuring electrical processes and possible numerical solution of this problem. Simulation of action potentials and membrane currents is important to understand the basic electrical processes in the cell. The result of this thesis is a program which simulates the voltage clamp method. Program can depict basic membrane currents and manages to resolve the values according to user-specified parameters. Supplement of this thesis contains detailed user manual.
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Design of mechanical, electrical construction and software equipment of autonomous cell "Ice crusher" for Industry 4.0 testbed.
Horák, Lukáš ; Benešl, Tomáš (referee) ; Pásek, Jan (advisor)
The goal of this bachelor thesis is design and construction of an autonomous cell on a production line with the purpose of being an „ice crusher“. The purpose of this cell is to be part of a Testbed. The cell will be controlled using a HMI touch panel and PLC 1200. Information about how much ice the cell should pour into a glass is stored in an NFC chip on the bottom of the glass. The first part of the thesis comprises of brief introduction to Industry 4.0 alongside with description of the default and current state of Industry 4.0 testbed. Another part describes mechanical and electrical design of the cell. Last part of the cell describes software of the cell alongside with description of testing and installing the cell to the testbed.
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